The small footprint and touchscreen control allows for easy laboratory setup with minimal investment of time, cost, and effort. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. together with a Molecular Devices plate. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a. Visualize cells with your microplate reader Imaging with the SpectraMax MiniMax 300 Imaging Cytometer mirrors the plate reading workflow on the SpectraMax i3x System. Visible absorbance -. Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. The resulting OD 426 values are visually represented above as a color map generated in the plate section of SoftMax. and it is unrealistic to expect one experiment to occupy a plate reader for multiple hours or days. Supplier: Molecular Devices. 7°C (gradient heating design). The user must ensure that the meniscus is comfortably above the light beam in standard cuvettes and that the sample chamber in a microcuvette is aligned properly with the beam. Users of Molecular Devices' SpectraMax® i3 Multi-mode reader can even add an optional. W×D×H. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. 2 Software Release Notes. A control well was set up to verify the accuracy of the standard curve. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. Step. in steps of 0. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader s with all SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. Molecular Devices launches custom organoid line expansion service using unique bioprocess technology for high-throughput applications. 6 kg (30 lbs. Step 4: Add detection antibody – Enzyme-conjugated detection antibody binds to a second site on the. Molecular Devices announced today the launch of the new SpectraMax ® iD5 Multi-Mode Microplate Reader, a feature-rich platform that expands the boundaries of scientific research. Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader. 3860 N First Street San Jose, CA 95134. SpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Cuvette Chamber The cuvette chamber is located to the right of the control panel. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution designed to expand the boundaries of research capabilities. Recombinant protein expression Nucleocapsid. Cell line development teams will realize savings on time and resources,Some plate readers handle label-free cell-based assays, such as PerkinElmer’s EnSpire® multimode reader, which in addition to absorbance, luminescence, fluorescence and AlphaLISA also supports Corning’s Epic® technology. NORTH AMERICA. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. Cellular Imaging Systems. Absorbance Plate Reader Comparison Chart. VMax . To maximize sensitivity of the assay, it is necessary to use optimal excitation and emission wavelengths. Use form “26003-02: Molecular Devices Plate Reader Plate Calibration Form” to record material and equipment information. A preconfigured. The plate was then re-read on the plate reader (Read B). The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. W×D×H. With the discontinuous kinetics feature in SoftMax Pro Software. Eight filter options provide simplified assay setup. ®100 μL of each endotoxin standard was added to triplicate wells of an LAL Reagent Grade Multi-well Plate. 5: Schematic of the back panel of a reader. Plate Reader. Step 1: Capture antibody binds to ELISA plate wells. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection reader. Find new and used lab equipment and shop a wide selection of products and vendors. The SpectraMax® M2 and M2e Systems from Molecular Devices are multi- detection microplate readers with dual- monochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). and fluorescence microplate readers—. Step 3: Wash microplate – Unbound material is washed away, leaving only the antigen of interest. Use all black plate. Laboratory automation workflows can help. 8×15×8. The plate reader optics are tunable for absorbance. Lamp source is a Xenon flash lamp (1 Joule/flash). A wide range of assays can be performed using a microplate reader including ELISAs, protein cell growth assays, nucleic acid and protein quantification, enzyme activity measurement, immunoassays, and screening of drug compounds and targets, among others. D SpectraMax ® M2 SpectraMax ® M2 e Multimode Plate Readers User Guide Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part #0112-0102 Rev. FLIPR. The adapter is required for optimum performa nce with standard 96-well and 384-. Hold down the green power button on the front of the instrument for 5 seconds or until the instrument power down. 과 같은 Parameter를 검사하여 평가할 수 있습니다. In this product video, see how the SpectraMax M5 quickly reads samples. Due to their ability to simultaneously measure multiple wells in a single run, fluorescence plate readers are useful for conducting various assays, such as quantitative assays, enzyme assays, and ELISAs. Molecular Devices' microplate readers. Built around a grating monochromator, the VersaMax™ Absorbance Microplate Reader allows for selection of up to two wavelengths at a time for absorbance detection in the visible wavelength range (340–850nm). The SpectraMax® Paradigm® Multi-Mode Microplate Reader measures absorbance, fluorescence, time-resolved fluorescence (including HTRF), fluorescence polarization, AlphaScreen®, AlphaLISA®, and luminescence assays for up to 1536-well plates. Molecular Devices SoftMax ® Pro software was used to analyze and present the data. Supplier: Molecular Devices. 40-plate magazine (pair) 0200-6017. Step 1: Open the SoftMax Pro IMAP FP protocol. Read one sample or up to 384 in a. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplateMicroplate Spectrophotometers. Company Info. The sample is typically placed in a cuvette through which light is sent horizontally. S. 4: Test tube cover. After reading the ELISA plate on both readers, each standard curve was nearly identical. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. It also allows for the elimination of standard curves. Cells were thawed and propagated according to the recommended method specified by the supplier. 9. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. Ratios for each sample were calculated as Read B/Read A. 0112-0115J 9 Chapter1:Introduction TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-Mode SpectraMax® Microplate Reader User Manual Download Page. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. 5032018C 8 Chapter1:Introduction TheEMax® PlusMicroplateReaderisafilter-basedabsorbancemicroplatereaderwitheight standardfiltersforawiderangeofapplications. This multi plate reader is excellent to work with for checking the U. Plate stacker and robot integration The Gemini EM Reader can be integrated with Molecular Devices’ StakMax® Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. Find our technical support information along with contact details. US HEADQUARTERS. Figure 1. We would like to show you a description here but the site won’t allow us. Reads standard cuvettes & 96-384 well plates from 190 to 1000 nm. Page 1 ™ VERSAmax Tunable Microplate Reader Operator’s Manual for Instruments with Serial Numbers Beginning with BN Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0084 Rev. Molecular Devices SpectraMax® microplate readers support a multitude of assays for nucleic acids and protein detection. PURPOSE. Shop now. The instrument is designed to regulate the temperature of the plate chamber from 5°C above ambient to 45°C. Optimize. and California) Tunable Microplate Reader 100-240V ~ 4A 50-60 HZ T4. The FLIPRTETRA, which debuts today at. The Molecular Devices FilterMax F5 (replacing our old Analyst plate reader) is a versatile instrument that offers detection based on fluorescence, fluorescence polarization, luminescence, absorbance, and time resolved FRET. Purchase is subject to Molecular Devices standard terms and conditions of sale. Microplate Reader Shelf. Read the plate on the SpectraMax M5, M5e, or FlexStation 3 reader using the optimized settings shown above. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. SpectraMax iD5 Multi-Mode Microplate Reader, a hybrid monochromator- and filter-based, five-mode plate reader with automatic filter identification and western blot capability. Fluorescence analysis was performed using a SpectraMax iD3 multi-mode plate reader (Molecular Devices LLC, U. Molecular Devices의 Microplate Reader 및 washer와 원활하게 호환되는 강력한 벤치탑 Microplate stacker입니다. Fluorescent Imaging Plate Reader (FLIPRTM ) technology to measure Ca2+ flux in cells was an important development in the early 1990’s and has played a significant role in. #6590-0118) Filter 665/10 (Molecular Devices cat. Prior to fluorescence analysis, the lid from the OmniTray plate was removed. In . APP Automated. Microplate spectrophotometers, also called microplate readers, allow researchers to analyze multiple samples in parallel using 6- to 1536-well formats, as opposed to single sample measurements. Molecular Devices' microplate readers. Register to download your free eBook today. Sale price $18,499. Molecular Devices SpectraMax Gemini XS Microplate Reader. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry leading SoftMax Pro. Find regional support. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. Absorbance values were read on the SpectraMax iD5 reader using a preconfigured BCA assay protocol (the SpectraMax iD3 reader is also suitable for these assays, as it has the same absorbance performance as the SpectraMax iD5 reader). The VersaMax Absorbance Microplate Reader allows for selection of up to two wavelengths at a time for absorbance detection in the visible wavelength range (340 nm - 850 nm). with the kit reagent and transferred to the plate reader for evaluation at the same time. The automated ELISA workflow has two positive outcomes. Page 1 of 8 . Please Inquire. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. Monthly Plate Verification Check (Absorbance Validation Plate) Note: Monthly Plate Verification Check is maintained by Quality Control. The user guide also covers the features, specifications, and software of the reader, as well as the installation and. Successful targets include calcium channels, chemokine receptors, and transiently transfected receptors. 0200-6262. For more sample throughput, both readers can be easily. Pre-defined protocols. Under "Category", select "Plate Type". 72 MB. Used. Page 1 of 8 . Supplier: Molecular Devices. 5 minutes (ValitaTiter) 15 minutes (ValitaTiter PLUS) Integrating ValitaTiter and Molecular Devices fluorescence polarization (FP) plate readers produces a seamless IgG titer assay setup and optimization. Instrument settings can be saved as a protocol file. The SpectraMax 190 reader can be integrated with Molecular . The SpectraMax ® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of. From plate readers to high-content imagers, Molecular Devices has the instruments you need to advance your discovery. The FlexStation 3 Reader combines Molecular Devices’ SpectraMax® M5e Microplate Reader performance with an integrated 8- or 16-channel pipettor into one compact benchtop reader. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. Feb 22, 2023Used. Our specialized plate readers for absorbance, fluorescence, and luminescence detection provide optimal sensitivity. Lastly, we demonstrate the utility of this novel fluorescence-based assay to evaluate the activities of. Weight. The AlphaScreen Detection Cartridges utilize a 680nm Laser Diode to provide a sensitive reading system for AlphaScreen assays. “Sensitivity is pretty much clutch for luminescence,” says Daly. The SpectraMax L reader is easily integrated with the optional StakMax microplate stacker from Molecular Devices for automated multiplate assays. 32 was diluted 10-, 100-,. This process should take about 3 - 5 minutes. 13. This document is provided to customers who have purchased Molecular Devices, Inc. Multimode microplate readers offer flexibility, and ease of use. PURPOSE. Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time-resolved fluorescence (TRF), FRET, and AlphaScreen. Choose the desired plate type, using the upper dropdownThe Molecular Devices Gemini EM is built on the Gemini platform delivering high quality top and bottom reading for both solution and cell based assays. Molecular Devices, LLC. Table of Contents. Additional high-quality filters to supplement your assay wavelength range requirements are available directly from Molecular Devices. What plates do you recommend for SpectraMax Plate Readers? Luminescence: Solid white plates for top reads and white plates with clear bottoms for bottom reads. 5 µL of each sample was pipetted into a 96-well. UV/Vis spectrophotometer & microplate reader with built-in cuvette port. The ultimate guide to microplate readers is an eBook that tells you everything you need to know about plate reader solutions from what a microplate reader is, to popular applications and technology, to GxP compliance and special tips and tricks to help maximize your. Expanded wavelength coverageOther readers with cell imaging include PerkinElmer’s EnSight™ Multimode Plate Reader and Molecular Devices’ SpectraMax i3x Multi-Mode Microplate Reader. Double-click the unknown device with the yellow warning icon. The FlexStation uses a Xenon flashlamp and monochromators for both excitation and emission light to allow the use of a wide range of. Calcium ions (Ca2+) play a key role in cellular homeostasis involving calcium channel and GPCR function, which plays a critical role in many disease pathologies. The touchscreen interface provides integrated instrument control, data display, and the ability to export results for statistical data analysis. Molecular DevicesThe Marker Expression Protocol is used to identify cell areas labeled with the fluorescein-conjugated anti-VCAM antibody. Your applications, your modes, your choice. When you flip the LM1 plate over for checking Bottom Read, remove the Purple Plate adapter. Place the microplate in the drawer. After centrifugation, the plate was immediately read on the M5e and i3x readers using the instrument settings listed in Table 1. Step 4: Incubate at room temperature for 1 hour, protected from light. ELISA Plate Readers / Microplate Readers. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the. The system also gives users the flexibility to choose wavelengths between 200 nm and 1000 nm. Molecular Devices provides the complete solution covering the instrument and software: • SpectraTest® ABS1, FL1, and LM1 Validation Plates for hardware validation of absorbance, fluorescence, and luminescence modes • IQ/OQ for all microplate readersMolecular Devices technical support. Changing Plate Hold-Down Devices 123 Cell Reservoir 125 Chapter 7: Calibration and Signal Test 127. The plate reader optics are tunable for absorbance, fluorescence intensity,. Product Detail. United States Phone: 800-635-5577 408-747-1700. SpectraMax® Microplate Readers and SoftMax® Pro Software. Monday to Friday 7am - 5pm Pacific Time. The SpectraMax® M2 and M2e Systems from Molecular Devices are multidetection microplate readers with dualmonochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). Versa Max. 00 USD. As Glazer explains, “Our readers have software that analyzes data as it’s acquired from a plate—doing both at the same time. Imaging with the MiniMax 300 Cytometer mirrors the plate reading workflow on the SpectraMax i3x System. These systems are hybrids that allow you to choose between. Endpoint, kinetic, spectrum. It was then read from the bottom on a SpectraMax ® i3 Multi-Mode Microplate Reader using a preconfigured protocol in SoftMax Pro Software with the settings indicated in Table 1. book Page 1 Tuesday, July 6, 2010 2:08 PM Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time-resolved fluorescence (TRF), FRET, and AlphaScreen. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modesRobust, high-value microplate readers that can run. Open the Protocol Library, locate the ‘Protein Quant’ folder, and open the pre-configured protocol ‘NanoOrange’. Page 1 of 8 . An IL-22 standard curve was prepared and a sandwich ELISA performed using the MultiWash+ plate washer in strip mode to wash the wells. Use form “26003-02: Molecular Devices Plate Reader Plate Calibration Form” to record material and equipment information. Table of Contents. Eight filters enable visible-wavelength absorption measurement applications such as protein quantification, cell viability and ELISA. The Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader is a dual-monochromator, multi-detection microplate reader with full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. Our extensive SpectraMax® line of user-friendly microplate readers – the industry’s most cited brand – provide great flexibility for labs conducting applications ranging from ELISAs to nucleic acid and protein quantitation, and include absorbance, fluorescence, luminescence and more ( Molecular Devices ) equipment, software, reagents, and consumables to use in the operation of such Molecular Devices equipment, software, reagents, and consumables. D SpectraMax ® M2 SpectraMax ® M2 e Multimode Plate Readers User Guide Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089. Narrow bandwidths deliver increased accuracy and enhanced peak resolution. Accurate and sensitive detection of nucleic acids and proteins are critical to many experiments. The SpectraMax® M2 and M2e Systems from Molecular Devices are multi- detection microplate readers with dual- monochromators, dual-mode cuvette ports, and top- and. 306 plate air cal. Learn how you can utilize microplate readers to drive landmark discoveries. The computer controlling the plate reader runs Windows XP. (a) Chemical structures of 1 (HADP) and its analogues 2–4. The purpose of this procedure is to set instructions in the proper use and handling of the Molecular Devices M5 Plate Reader. SpectraMax Absorbance, Fluorescence. The SpectraMax ® i3x Platform from Molecular Devices is a multi-mode microplate reader that evolves with your future needs and offers an unlimited breadth of application possibilities. The 1/absorptivity value for double stranded DNA at 260 nm is commonly assumed. The SpectraMax® M3 Multi-Mode Microplate Reader dual monochromatic optics allows the widest range of applications to be utilized for bioresearch applications all without the need to change filters, and provides three modes of detection in one platform. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Are you looking for a versatile and affordable microplate reader that can perform various assays and measurements? Check out the FilterMax F3 and F5 Multi-Mode Microplate Readers, which offer absorbance, fluorescence, luminescence, and more read modes with high performance and reliability. They provide absorbance, fluorescence, glow luminescence, and other read modes for programmable endpoint, kinetic, multiple-wavelength linear scan and area scan measurements. This assay kit is optimized for SpectraMax microplate readers from Molecular Devices, with a pre. Narrow bandwidths deliver increased accuracy and enhanced peak resolution. The concentration of total free thiols in the protein. ABS2, FL1, and LM1 Validation Plates sent to Molecular Devices for recertification at our ISO 17025 accredited lab are disassembled, cleaned, calibrated, an then returned with a new certificate of calibration. Detection cartridge modularity allows for easy addition of new read modes within minutes without. Expand the Port (COM & LPT) Right click on the USB Serial Port (COMx) and select Properties. tools to expand the boundaries of their research. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. #4TI-0655) Microplates. An example of an upgradeable multi-mode plate reader is the SpectraMax M5e, a five-mode reader that is part of the same line as the first microplate reader used in space, thanks to its durability. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 1. Plate reader. 0 EU/mL, but the additional standard concentration (0. 0 × 107) RLU 0. Search. Samples with volumes as low as 2 µL can be measured. This integrated system provides users with a multi-detection platform capable of increasing the liquid handling throughput and flexibility for biochemical- and cell. Since cells are nearly confluent, the protocol. 2. This eBook shows how to see more and do more with an imaging cytometer that enables rapid imaging and cell analysis. Detection of KASP genotyping reactions For the second test, the validation kit contained 36 known samples, including 33 DNA samples and 3 no-template controls (NTC). Cellular Imaging Systems. The SpectraMax® ABS and ABS Plus absorbance microplate readers provide the flexibility, sensitivity, and convenience for a wide range of assays such as ELISAs, microbial. Detector (s) PMT and/or photodiode. OPTICS Mirrored optics focus the light into the sample volume, and cutoff filters are used to. The adapter is required for optimum performa nce with standard 96-well and 384-. Turn on Plate Reader and wait for it to complete its start-up routine. 1-1. Results shown are from the SpectraMax M5 microplate reader, but similar results are obtained with other Molecular Devices readers with fluorescence detection mode (data not shown). Chirag Patel, a graduate student at Johns Hopkins, reviewed Molecular Devices' SpectraMax M5 Multi-Mode Microplate Reader and found it an excellent resource for his lab's research on mTOR signaling in CD8 T cells. This five-mode microplate reader with automatic filter identification and western blot capability is the complete laboratory solution to help scientists increase. 340 PC384. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance in one multimode reader package. begin reading microplates with seven mouse clicks. With optimized reagents, option to operate the. GxP regulated industry assessments of microplate readers. No accessories are needed and SoftMax® Pro Software. Set up template in SoftMax Pro and read plate on the SpectraMax M5 microplate reader: Note: IMAP FP protocols for use with FAM- and TAMRA-labeled substrates are available in SoftMax Pro 5 in the Binding Assays protocol folder. A. Note that this procedure is only for the SpectraMax L model. Additional high-quality filters to supplement your assay wavelength range requirements are available directly from Molecular Devices. $2,800. This document is provided to customers who have purchased Molecular Devices, Inc. Select the Driver tab. Fill a clean plate with water. 1. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader. The SpectraMax® QuickDrop™ UV-Vis Spectrophotometer quantifies very small amounts of DNA, RNA, oligos, and proteins. FLIPR Tetra systems are fluorescence- and luminescence-based microplate readers with an integrated 1536-, 384- or 96-pipettor. (“Molecular Devices”) equipment, software, reagents, and consumables to use in the. Reader Documentation Version No. 1. Price Get Quote. The plate reader temperature was set to 37°C and allowed to reach 37°C before proceeding. Step 1: Turn on your microplate reader. (1) Repertoires of SARS-CoV-2 epitopes targeted by antibodies vary according to severity of COVID-19 Virulence December 31, 2022 David J. Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. SoftMax Pro Software. . . FilterMax F3 and F5 Multi-Mode Microplate Readers are filter-based microplate readers offering fluorescence polarization, time-resolved fluorescence, glow luminescence, and other read modes for programmable endpoint, kinetic, multiple-wavelength linear scan and area scan measurements. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. Molecular Devices SpectraMax Gemini XS Microplate Fluorometer. Read the microplate • Make sure the purple plate adapter (if required for your plate reader) is in the microplate reader drawer. Supplier: Molecular Devices. Sensitivity and dynamic range are demonstrated by the high Z’ factors obtained on the SpectraMax. • Fluorescence Polarization (FP-FLUO) Detection Cartridge (Molecular Devices cat. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 1. 0 . #0200-7009) Optimal instrument settings for ValitaTiter (P/N VAL003 or VAL013, left table) and ValitaTiter Plus (P/N VAL004 or VAL014, right table) assays on the SpectraMax i3x reader are shown below. V. AlphaScreen assays cannot be done on the SpectraMax M series, Gemini, and SpectraMax iDx (iD3/iD5) readers. By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. Read height should be optimized forVmax® plate reader specifications. ) Enables data acquisition in endpoint, kinetic, and spectral scan modes at wavelengths across the UV-visible wavelength range, with the only temperature-independent method of pathlength correction. Expand the Port (COM & LPT) Right click on the USB Serial Port (COMx) and select Properties. Luminescence was read every five minutes for five hours with three seconds of orbital shaking before each read. Luciferase reporter assays are widely used because of their sensitive detection capacity and broad dynamic range. Molecular Devices. We obtained three HEK-293 cell lines from Clontech, each stably transfected with a different fluorescent protein. Tests every subsystem of the reader or . An industry-renowned instrument for monitoring GPCRs and ion channels. 0A Sunnyvale, CA Made in USAFilter 320/100 (available from Molecular Devices) Filter 616/10 (Molecular Devices cat. July 17, 2023. 001 EU/mL) was added in order to test the sensitivity of the SpectraMax i3 reader. Plate stacker and robot integration The Gemini EM Reader can be integrated with Molecular Devices’ StakMax® Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. Sunnyvale, Calif. 2. Description 1. 00 USD. • Fluorescence Polarization (FP-FLUO) Detection Cartridge (Molecular Devices cat. SpectraMax® Absorbance Microplate Readers can run both cuvette-based and microplate reader applications on the same instrument. A control well was set up to verify the accuracy of the standard curve. 9000-0756. The plate was then read on Molecular Devices microplate readers with luminescence detection mode (Read A). SpectraMax Microplate Readers. . Examples include ELISAs for quantitation of cytokines and protein concentration determination using the Bradford assay. This offer includes SpectraMax i3x, SpectraMax. In addition, the iD5 reader measures TRF and FP and. Detection cartridge modularity allows for easy addition of new read modes within minutes without. • 0. The Gemini XPS Reader can be integrated with Molecular Devices’ StakMax ® Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. Enable more assay conditions Define individual reagents to be delivered to each well reducing reagent consumption and allowing for more assay conditions to be explored on a single microplate. 0) OD (0. plate shaker and incubate for 2 to 5 minutes at room temperature, protected from light. SoftMax Pro 7. Wait 20 seconds. Published Dec 16, 2020 | Updated May 19, 2023. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes wi. As new assays are developed or your needs change, simply add a detection cartridge like ScanLater™ Western Blot System or the SpectraMax® MiniMax™ Imaging Cytometer option as opposed to buying a brand new system. Number of Citations*: 8350. 25 to 3. Firmly grasp the snap connector beside the snap, then push the snap down. The FilterMax™ F3 and F5 Multi-Mode Microplate Readers offer a wide set of features at an affordable price-to-performance ratio. Fluorescence Polarization (FP) Glow Luminescence (Lumi) The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast,. Powered by a new, optional high-speed EMCCD camera and the new Peak Pro 2 software module, the new FLIPR® Penta High-Throughput Cellular Screening System allows you to measure and analyze peaks of human-derived induced pluripotent stem cells (hiPSCs), differentiated into. 9. Easy-to-use touchscreen allows you to quickly set up your experiments, use preconfigured analysis methods, and view tutorial videos. S. SpectraMax® Plus 384 Absorbance Plate Reader, Molecular Devices. 1. Uncontrolled Copy for Training and Reference Purposes Only . The ultimate guide to microplate readers is an eBook that tells you everything you need to know about plate reader solutions from what a microplate reader is, to popular applications and technology, to GxP compliance and special tips and tricks to help maximize your. While many companies make instruments to detect and measure luciferase activity, identifying the right instrument for you can be difficult because of their high degree of feature variability. 5 sec. Turn the reader on and secure the USB cable. edu. This process should take about 3 - 5 minutes. We would like to show you a description here but the site won’t allow us. For nearly 30 years, Molecular Devices has provided scientists with . Description: ScanLater* Western Blot Detection Cartridge, enables the first western blot detection capability to a microplate reader, Time-resolved fluorescence detection, Xenon flash lamp excitation source. Built-in near-field communication (NFC) reader enables you to pull up your custom protocols and results with a single tap, saving you precious time. SpectraMax M5. FlexIII. Versa. S. For the FlexStation 3 Reader - Keep the Black Plate Adapter in place fro both Top and Bottom reads. please click here. 0200-6263. 0 . Flexibility to read a variety of plate formats. Instrument settings can be saved as a protocol file. Summary of Contents for Molecular Devices SpectraMax M2. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes wi. The opaque walls prevent stray light from being detected from neighbouring. Cet appareil ISM est confomre à la norme NMB-001 du Canada. Read times. BMG Labtech - THERMOstar. This reader utilizes Molecular Devices' patented PathCheck® Sensor to measure the depth (optical pathlength) of samples in a microplate. The plate was incubated at room temperature for 50 minutes or 2. ; Page 2 Molecular Devices equipment, software, reagents, and consumables. Molecular Devices: EMax Plus: SpectraMax 190: SpectraMax 340PC384: SpectraMax Plus384: VERSAmax:. Multimode Microplate Readers. Every step is optimized to simplify analysis and reporting to support our microplate readers. 0A Sunnyvale, CA Made in USA Microplate readers read biological, chemical or physical events that occur on a microtiter plate. The adapter is required for optimum performa nce with standard 96-well and 384-.